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KMID : 1094720120170020298
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 2 p.298 ~ p.308
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Proteome analysis on lethal effect of l 2 in the sex-linked balanced lethal strains of silkworm, Bombyx mori
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Chen Jine
Niu Baolong
Wang Yongqiang
Liu Yan
Liu Peigang
Meng Zhiqi
Zhong Boxiong
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Abstract
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The sex-linked balanced lethal (SLBL) strains of silkworm serve as an effective system for sex-control in silkworm. To gain comprehensive insight into the effect of one sex-linked balanced lethal gene l 2, comparative proteomic analysis was carried out between the survival embryos (W+l1Zl1+l2) and lethal embryos (W+l1Z+l1l2) before the lethal stage. The lethal stage of l 2 was confirmed by observing the typical dead embryo morphology. The two genotype embryos before lethal stage were distinguished using polymorphic simple sequence repeats (SSR) markers closely linked to l 2 on the sex chromosome. Finally, 11 differentially expressed protein spots were successfully identified by MALDI-TOF/TOF mass spectrometry (MS). Among them, only 1 protein identified as heat shock protein 20.4 (HSP20.4) was up-regulated in the lethal embryos, while the other 10 were down-regulated. The up-regulation of HSP20.4 suggests that there may be abnormal polypeptides produced in the lethal embryos. The gene ontology (GO) annotation indicated those down-regulated proteins are involved in important biological processes including embryo development, nucleoside metabolism, tRNA splicing, translation and protein folding. The biological pathway analysis showed that those down-regulated proteins are mainly involved in spindle assemblage and morphogenesis. Based on our results, we suggest that the l 2 may be the mutant expressing abnormal polypeptides. Its expression has a negative effect on mitosis and morphogenesis processes. The death of the embryos may be caused by the accumulation of abnormal polypeptides and the handicap of cell proliferation and morphogenesis.
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KEYWORD
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embryo, 2-DE, MALDI-TOF/TOF, sex-linked balanced lethal, silkworm
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